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Updated: Jun 10, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Cautionary note: linewidth effect in dynamic NMR
1Dept. of Chemistry, University of Saskatchewan, Saskatoon, Canada. ohn.weil@usask.ca
Abstract:
In using NMR spectroscopy to evaluate the effects of kinetic processes taking place in the solute molecules being studied, one must estimate the inherent width w of each line, which is hidden from view throughout the kinetic thermal region due to the line-width broadening effected by the processes. We have herein built a self-consistent model function w(T) to attempt to evaluate the variation in the Arrhenius activation energy obtained with the model parameters present in w(T). As it turns out, a sizable number of such line-width parameters yields excellent spectral fits, and these lead to a considerable range of possible activation energies.
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