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Updated: Jun 10, 2026
12:34
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
Two-dimensional gel electrophoresis in proteomics: Past, present and future
Thierry Rabilloud1, Mireille Chevallet, Sylvie Luche
1CNRS UMR5092, Biochemistry and Biophysics of Integrated Systems, CEA Grenoble, iRTSV/BSBBSI, Grenoble, France. Thierry.Rabilloud@cea.fr
Journal of Proteomics
|August 6, 2010
Summary
Two-dimensional gel electrophoresis (2D-PAGE) was crucial for proteomics development. This review highlights 2D-PAGE's enduring advantages and optimal use in modern proteomics workflows.
Area of Science:
- Proteomics
- Biochemistry
- Analytical Chemistry
Background:
- Two-dimensional gel electrophoresis (2D-PAGE) played a foundational role in the early development of proteomics.
- Initially, 2D-PAGE was the primary separation technique before other methods emerged.
Observation:
- While no longer the sole technique, 2D-PAGE's historical significance in proteomics is undeniable.
- The review traces the evolution of proteomics from the era of 2D-PAGE dominance.
Findings:
- 2D-PAGE possesses inherent limitations in modern proteomics applications.
- However, its strengths, including robustness, high resolution, and separation of intact proteins, remain valuable.
Implications:
- Understanding 2D-PAGE's advantages allows for its strategic application in specific proteomics workflows.
- Future applications leveraging 2D-PAGE's unique capabilities in proteomics are explored.
Related Concept Videos
01:22Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:27SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
01:20Electrophoresis: Overview
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...