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Updated: Jun 10, 2026
Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
Published on: June 28, 2024
Detection of Newcastle disease virus with quantum dots-resonance light scattering system
Qiang Ma1, Wei Yu, Xingguang Su
1Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun, Jilin 130012, China.
Abstract:
A sensitive QDs-based RLS assay method for the detection of Newcastle disease virus (NDV) antibody has been developed. CdTe quantum dots (QDs) were conjugated with Newcastle disease virus and used as RLS-based probes to detect NDV antibody. The electrostatic interaction between CdTe QDs and NDV resulted in enhanced resonance light scattering (RLS) signal characterized at 555 nm. Upon the addition of NDV antibody, QDs-NDV formed dispersive immunocomplex that can decrease the RLS signal. The decreased RLS intensity at 555 nm (DeltaI(RLS)) was linearly proportional to the concentration of NDV antibody (C(anti-NDV)) in the range of 0.5-50 ng/mL, with correlation coefficient of 0.974 and detection limit of 0.1 ng/mL under the optimization conditions. The proposed method was applied to the determination of NDV antibody in spiked samples with satisfactory results.
