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Related Concept Videos

RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...

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Related Experiment Video

Updated: Jun 10, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

Folding and finding RNA secondary structure.

David H Mathews1, Walter N Moss, Douglas H Turner

  • 1Department of Biochemistry and Biophysics and Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.

Cold Spring Harbor Perspectives in Biology
|August 6, 2010
PubMed
Summary
This summary is machine-generated.

Discovering and folding RNA sequences is crucial. New computational methods combine thermodynamics and experimental data for rapid RNA identification and structure prediction, aiding biological insights and therapeutic design.

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Related Experiment Videos

Last Updated: Jun 10, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

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06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Molecular Biology

Background:

  • The rapid growth of genomic sequence data necessitates efficient methods for identifying and characterizing functional RNA molecules.
  • Understanding RNA structure and function is key to deciphering biological processes and developing novel therapeutics.

Purpose of the Study:

  • To review methods for automating the discovery and folding of RNA molecules.
  • To highlight the integration of computational algorithms with experimental data for RNA structure determination.
  • To discuss the implications of RNA discovery for evolutionary studies, biology, and therapeutic design.

Main Methods:

  • Algorithms coupling thermodynamics with chemical mapping, Nuclear Magnetic Resonance (NMR), and sequence comparison for RNA folding and discovery.
  • Utilizing predicted free energies and experimental data to refine RNA secondary structure determination.
  • Employing secondary structure information to model three-dimensional RNA structures.

Main Results:

  • New functional noncoding RNAs can be identified by integrating sequence comparison with predicted folding free energies.
  • A combination of experimental data and sequence comparison accelerates the determination of RNA secondary structures.
  • The process allows for the subsequent modeling of three-dimensional RNA structures, exemplified by a retrotransposon domain.

Conclusions:

  • Automated methods enhance the exploitation of vast sequence databases for RNA discovery and structural analysis.
  • The accurate prediction of RNA secondary and tertiary structures provides critical insights into RNA function and evolution.
  • This approach has significant potential for advancing our understanding of biological systems and designing RNA-based therapeutics.