Related Experiment Video

Updated: Jun 10, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

Comparative genomic analyses identify the Vibrio harveyi genome sequenced strains BAA-1116 and HY01 as Vibrio

Baochuan Lin, Zheng Wang, Anthony P Malanoski

    Environmental Microbiology Reports
    |August 6, 2010
    PubMed

    Insights

    Accurate identification of Vibrio harveyi and related species is challenging. This study reveals significant misidentifications within the Harveyi clade, including novel species, using genomic analyses.

    Area of Science:

    • Microbiology
    • Genomics
    • Marine Biology

    Background:

    • The Harveyi clade, including Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus, comprises significant marine pathogens.
    • Genetic and phenotypic similarities make discriminating between species within the Harveyi clade difficult, leading to misidentifications.

    Purpose of the Study:

    • To investigate the genetic basis for species-level discrimination challenges within the Harveyi clade.
    • To accurately identify and classify strains within the Harveyi clade using genomic approaches.

    Main Methods:

    • Microarray-based comparative genomic hybridization (CGH) was employed to compare genomes of Harveyi clade members.
    • Multilocus sequence analyses (MLSA) were utilized to assess genetic relatedness and species boundaries.
    • Strains were isolated from diverse environmental sources, including marine environments and hosts.

    Main Results:

    • Two previously identified Vibrio harveyi strains were reclassified as Vibrio campbellii, highlighting significant misidentification.
    • A substantial proportion (28%) of tested strains were found to be misidentified.
    • Approximately 42% of the misidentified strains potentially represent a novel species within the Harveyi clade.

    Conclusions:

    • Genomic analyses, specifically CGH and MLSA, are effective tools for accurately distinguishing closely related species within the Harveyi clade.
    • This study corrects several taxonomic misclassifications and underscores the importance of rigorous genomic identification for understanding pathogen prevalence and ecology.

    More Related Videos

    Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates07:58

    Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

    Published on: May 30, 2017

    Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview06:44

    Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview

    Published on: May 28, 2007

    Related Concept Videos

    Bacteriophages of the Human Virome01:23

    Bacteriophages of the Human Virome

    Bacteriophages are found throughout the human body. They may even outnumber eukaryotic viruses, forming an important and dynamic component of the human virome. Indeed, phages represent the most abundant viral entities, with densities in the gut reaching up to 10⁹ particles per gram of fecal matter, and many belonging to orders such as Caudovirales and Microviridae, while a substantial proportion remains unclassified as viral “dark matter.”Lysogeny and Genetic ExchangeIn the gut, bacteriophages...
    Bacterial Gastroenteritis01:18

    Bacterial Gastroenteritis

    Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
    Bacterial Phylum Verrucomicrobiota01:26

    Bacterial Phylum Verrucomicrobiota

    The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
    Determinants of Bacterial Pathogenicity and Virulence01:20

    Determinants of Bacterial Pathogenicity and Virulence

    Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
    Bacterial Phylum Proteobacteria01:26

    Bacterial Phylum Proteobacteria

    Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
    DNA Bacteriophages01:26

    DNA Bacteriophages

    Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...