Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 10, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
15:31

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

Forward RNAi screens in human stem cells.

Christine Karlsson1, Jonas Larsson, Aurélie Baudet

  • 1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lithography-Free Water Stable Conductive Polymer Nanowires.

Nano letters·2025
Same author

TOPORS E3 ligase mediates resistance to hypomethylating agent cytotoxicity in acute myeloid leukemia cells.

Nature communications·2024
Same author

Functional and molecular profiling of hematopoietic stem cells during regeneration.

Experimental hematology·2023
Same author

GATA2 mitotic bookmarking is required for definitive haematopoiesis.

Nature communications·2023
Same author

Ciclopirox ethanolamine preserves the immature state of human HSCs by mediating intracellular iron content.

Blood advances·2023
Same author

Engineered human Diamond-Blackfan anemia disease model confirms therapeutic effects of clinically applicable lentiviral vector at single-cell resolution.

Haematologica·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Researchers developed a new RNA interference (RNAi) screening method for human hematopoietic stem cells. This technique aids in understanding stem cell regulation and identifying targets for stem cell expansion therapies.

Area of Science:

  • Stem Cell Biology
  • Cancer Biology
  • Molecular Genetics

Background:

  • Understanding somatic stem cell self-renewal and differentiation is crucial for stem cell biology and cancer research.
  • Hematopoietic stem and progenitor cells are key targets for studying these regulatory mechanisms.

Purpose of the Study:

  • To describe a novel RNA interference (RNAi)-based screening method applicable to primary human hematopoietic stem and progenitor cells.
  • To establish a new tool for dissecting regulatory mechanisms in stem cells.

Main Methods:

  • Utilized pooled lentiviral shRNA libraries for RNAi-based screening.
  • Employed selection-based screening in primary human hematopoietic stem and progenitor cells.
  • Developed a method suitable for complex genetic screens.

More Related Videos

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Related Experiment Videos

Last Updated: Jun 10, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
15:31

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Main Results:

  • Demonstrated the feasibility of RNAi screening in primary human hematopoietic stem and progenitor cells.
  • Established a method for complex, selection-based screens using pooled lentiviral shRNA libraries.
  • Validated the screening approach as a tool for dissecting regulatory mechanisms.

Conclusions:

  • The described RNAi screening method is a valuable new tool for stem cell research.
  • This approach can identify gene targets and modifiers for ex vivo stem cell expansion strategies.
  • The method has broad applicability for dissecting regulatory mechanisms in various somatic stem cell types.