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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Published on: April 28, 2019
Morphological changes in vero cells postinfection with dengue virus type-2
Seema Zargar1, Tanveer A Wani, S K Jain
1Department of Genetics, King Faisal Hospital and Research Centre, Riyadh 11211, Saudi Arabia. seemazargar@yahoo.com.
Microscopy Research and Technique
|August 6, 2010
Summary
Dengue virus infection alters cell structures, with high multiplicities causing cell death. The nucleus and mitochondria appear key in how the dengue virus spreads within cells.
Area of Science:
- Virology
- Cell Biology
- Pathology
Background:
- No specific antiviral treatments exist for dengue virus.
- Understanding dengue virus interaction with host cells is crucial for controlling propagation.
Purpose of the Study:
- To investigate the ultrastructural changes in Vero cells upon Dengue virus type-2 infection.
- To determine the effect of different multiplicities of infection (m.o.i.) on cellular morphology and survival.
Main Methods:
- Vero cells were infected with Dengue virus type-2 at varying multiplicities of infection (m.o.i. < 10, m.o.i. > 10, m.o.i. = 100).
- Ultrastructural analysis was performed at different time intervals post-infection (30 min, 1-12 h.p.i., 24 h.p.i.).
Main Results:
- M.o.i. < 10 allowed for optimal observation of morphological changes.
- M.o.i. > 10 led to apoptosis, while m.o.i. = 100 resulted in cell necrosis.
- Significant changes observed included nuclear membrane disintegration, cytoplasmic alterations, mitochondrial damage, and dispersed chromatin, suggesting organelle dysfunction.
Conclusions:
- Dengue virus infection induces significant ultrastructural damage to host cells, including nucleus and mitochondria.
- Mitochondrial dysfunction and changes in nuclear structure may play a role in dengue virus dissemination.
