Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chlorine isotopes constrain a major drawdown of the Mediterranean Sea during the Messinian Salinity Crisis.

Nature communications·2024
Same author

Pathogenetic and diagnostic significance of microRNA deregulation in peripheral T-cell lymphoma not otherwise specified.

Blood cancer journal·2014
Same author

Cluster phases of decorated micellar solutions with macrocyclic ligands.

The journal of physical chemistry. B·2013
Same author

Persistent agmination of lymphomatoid papulosis: an ongoing debate.

Dermatology (Basel, Switzerland)·2012
Same author

Partial nodal involvement by marginal zone lymphoma. Use of IGK gene rearrangement analysis in diagnostic work-up.

Pathologica·2011
Same author

β-Connectin studies by small-angle x-ray scattering and single-molecule force spectroscopy by atomic force microscopy.

Physical review. E, Statistical, nonlinear, and soft matter physics·2011
Same journal

Fiber-optic triggering of a two-stage high-current linear transformer driver with laser energy below 100 μJ.

The Review of scientific instruments·2026
Same journal

Optimization of laboratory-scale x-ray absorption spectroscopy (XAS) apparatus for nuclear fuel research.

The Review of scientific instruments·2026
Same journal

Compressed multi-scale entropy and its application in mechanical fault diagnosis.

The Review of scientific instruments·2026
Same journal

Bidirectional drive and multi-resolution adjustment across frequency bands in inertial impact piezoelectric motors via multimodal resonant vibration.

The Review of scientific instruments·2026
Same journal

A magnetic field sensor based on flaky Terfenol-D material and dual fiber grating.

The Review of scientific instruments·2026
Same journal

A novel E-field eight-way cavity combiner for high-power S-band applications.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2026

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

Intermittent contact hydration scanning probe microscopy.

G Aloisi1, F Bacci, M Carlà

  • 1Department of Chemistry, Via della Lastruccia 3, University of Florence, I 50019 Sesto Fiorentino (FI), Italy.

The Review of Scientific Instruments
|August 7, 2010
PubMed
Summary
This summary is machine-generated.

Hydration scanning probe microscopy offers a new way to image hydrophilic insulators and biological molecules. Oscillating the probe improves image stability by controlling water meniscus formation.

More Related Videos

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
11:59

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Published on: September 27, 2017

Related Experiment Videos

Last Updated: Jun 10, 2026

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
11:59

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Published on: September 27, 2017

Area of Science:

  • Surface science
  • Nanotechnology
  • Electrochemistry

Background:

  • Hydration scanning probe microscopy (HSPM) images hydrophilic surfaces using electrochemical current through a hydration layer.
  • HSPM can image insulators and biological molecules like DNA, but image stability is challenged by water meniscus formation.
  • Existing methods struggle with stable and reproducible imaging due to critical operating conditions.

Purpose of the Study:

  • To investigate meniscus formation and breakup in HSPM.
  • To determine optimal oscillation parameters for stable HSPM imaging.
  • To compare oscillating probe mode with continuous contact mode.

Main Methods:

  • Investigated meniscus formation and breakup dynamics.
  • Applied high-frequency probe oscillation, similar to tapping atomic force microscopy.
  • Analyzed probe-hydration layer interaction under varying oscillation amplitudes and frequencies.

Main Results:

  • High-frequency probe oscillation effectively controls water meniscus formation.
  • Optimized oscillation parameters lead to more stable and reproducible HSPM images.
  • Oscillating mode demonstrates advantages over the continuous contact mode for specific imaging conditions.

Conclusions:

  • Probe oscillation is a viable method to overcome meniscus instability in HSPM.
  • This technique enhances the imaging capabilities for hydrophilic insulators and biological samples.
  • Further research can refine oscillation parameters for advanced nanoscale imaging applications.