Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<b>A new species of <i>Parapsestis</i> Warren, 1912 (Lepidoptera, Drepanidae, Thyatirinae) from Yunnan, China with a key to species of the genus</b>.

Zootaxa·2026

Same author

Dynamic Analytical and Experimental Study of Wearable Thermoelectric Devices for Thermal Tactile Feedback.

Micromachines·2026

Same author

Chang'E-6 reveals solar wind-dependent H<sup>-</sup> ions on the Moon.

Science advances·2026

Same author

Bioinspired Tribologically Induced Liquid Metal Nanoparticle Coatings with Super-Lyophobic Characteristics for Soft Robotics.

Small (Weinheim an der Bergstrasse, Germany)·2025

Same author

Bow shock oscillations of Mars under weakly disturbed solar wind conditions.

Nature communications·2025

Same author

Multicomposition 3D Printing Using Tunable Ecoflex/Nanosilica Inks for Stretchable Electronics.

ACS omega·2025

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

New method for comparing DNA primary sequences based on a discrimination measure.

Jie Feng¹, Yong Hu, Ping Wan

¹School of Mathematical Sciences, Capital Normal University, Beijing 100048, China. fengjie0536@163.com

Journal of Theoretical Biology

|August 7, 2010

Summary

This summary is machine-generated.

We developed a novel DNA sequence comparison method using a unique distance measure. This automatic approach, based on primitive discrimination substrings, efficiently constructs phylogenetic trees without multiple alignments.

More Related Videos

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

A Practical Guide to Phylogenetics for Nonexperts

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

A Practical Guide to Phylogenetics for Nonexperts

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

Area of Science:

Bioinformatics
Computational Biology
Molecular Biology

Background:

Comparing DNA primary sequences is fundamental to understanding biological relationships.
Existing methods often rely on multiple sequence alignments, which can be computationally intensive and complex.
There is a need for efficient, automated methods for DNA sequence similarity analysis.

Purpose of the Study:

To introduce a novel, automatic approach for comparing DNA primary sequences.
To present a new measure of pairwise distances for sequence similarity analysis.
To demonstrate the method's utility in constructing phylogenetic trees.

Main Methods:

Developed a new distance measure based on primitive discrimination substrings between sequences.
Defined a discrimination measure DM(S, Q) for pairwise sequence similarity.
The method operates fully automatically, eliminating the need for multiple sequence alignments.

Main Results:

Successfully constructed phylogenetic trees using two independent datasets.
The proposed method demonstrated efficiency and power in sequence comparison.
The approach provides a robust alternative for analyzing DNA sequence relationships.

Conclusions:

The novel discrimination measure offers an effective and automatic way to compare DNA sequences.
This method is suitable for applications such as phylogenetic tree construction.
The approach is efficient and powerful for large-scale genomic analyses.