In vitro interactions between bone marrow stromal cells and hippocampal slice cultures

Bone marrow stromal cells (BMSCs) are capable of differentiating into various cell types including brain cells. Several groups have also demonstrated trophic effects of MSC grafts in experimental ischemia models. However, the underlying molecular mechanisms of these effects are not fully understood. We developed an "in vitro graft model" which consisted in a coculture of GFP-expressing BMSCs and hippocampal organotypic slice cultures. Total marrow cells (MCs) or BMSCs after one (BMSC(1P)) or five passages (BMSC(5P)) were transplanted on hippocampal slices. During the 10 days of our experiments, MCs and BMSC(1P) migrated toward the tissue, but their total number remained constant. Conversely, the number of BMSC(5P) decreased over the 10 days of the experiment, and no migration could be detected. Using immunohistochemistry, we observed that the hippocampal slices induced the expression of neural antigens in very few grafted cells, but MCs and BMSC(1P) improved the conservation of the hippocampal slice culture. Similar experiments using BMSC(5P) did not produce any significant change. We conclude that the number of passages greatly influence BMSCs survival rate, migration and neuroprotective capacities.