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Updated: Jun 10, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Primer-free aptamer selection using a random DNA library.

Weihua Pan1, Ping Xin, Susan Patrick

  • 1Department of Pathology, Hershey Medical Center, Pennsylvania State University, PA, USA.

Journal of Visualized Experiments : Jove
|August 7, 2010
PubMed
Summary
This summary is machine-generated.

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We developed a novel primer-free aptamer selection method that simplifies procedures and eliminates primer interference. This new approach enables efficient selection of high-affinity aptamers for various targets, including the melanoma marker S100B.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Aptamers are oligonucleotide-based therapeutics with antibody-like binding affinities.
  • Traditional SELEX methods often face challenges due to fixed primer sequences impacting aptamer identification.
  • Existing modifications to SELEX have limitations in library diversity and amplification efficiency.

Purpose of the Study:

  • To develop a simplified and effective primer-free (PF) SELEX method.
  • To eliminate primer-interference issues inherent in standard SELEX protocols.
  • To demonstrate the efficacy of PF SELEX in selecting high-affinity aptamers against specific targets.

Main Methods:

  • Developed a novel primer-free (PF) selection method for aptamer identification.

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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Related Experiment Videos

Last Updated: Jun 10, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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  • Purified the central random region of the library, bound it to the target, and then re-amplified.
  • Selected aptamers against S100B, a protein marker for melanoma, as a proof of concept.

    • Main Results:

    • The PF SELEX method significantly simplifies SELEX procedures.
    • Primer-interference problems were effectively eliminated.
    • Selected aptamers against S100B exhibited dissociation constants (Kd) in the 10(-7) - 10(-8) M range.
    • Demonstrated successful application of selected aptamers in a sandwich binding format.

    Conclusions:

    • The developed primer-free SELEX method offers a streamlined and efficient approach for aptamer discovery.
    • This method overcomes limitations of traditional SELEX, enabling robust aptamer selection.
    • The selected aptamers show high affinity and functionality, highlighting the potential of PF SELEX for diagnostic and therapeutic applications.