Related Experiment Video
Updated: Jun 10, 2026
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
CD25 appears non essential for human peripheral T(reg) maintenance in vivo
Marie-Ghislaine de Goër de Herve1, Emmanuel Gonzales, Houria Hendel-Chavez
1Unité d'Immunologie Biologique, CHU Bicêtre, Le Kremlin-Bicêtre, France.
Plos One
|August 7, 2010
Summary
Basiliximab, an anti-CD25 antibody, did not deplete regulatory T cells (Tregs) in pediatric liver transplant patients. However, altered Treg function was suggested by increased food allergies post-transplant.
Area of Science:
- Immunology
- Transplantation immunology
Background:
- Interleukin-2 (IL-2) is crucial for regulatory T cell (Treg) survival in mice.
- Basiliximab, an anti-CD25 antibody, is used therapeutically in organ transplantation.
Purpose of the Study:
- To investigate the effect of basiliximab on Treg maintenance and function in pediatric liver transplant recipients.
- To determine if CD25 blockade impacts peripheral Treg survival in humans.
Main Methods:
- Flow cytometry was used to analyze FoxP3+ CD4 T cells before and after liver transplantation.
- Patients received basiliximab, and Treg markers (CD25, FoxP3, IL-2Rbeta, IL-2Rgamma) were assessed.
Main Results:
- Basiliximab treatment did not deplete Tregs; FoxP3+ cell proportion and expression remained unchanged.
- IL-2Rbeta expression increased on Tregs post-basiliximab, while other IL-2 receptor subunits showed no significant change.
- Despite stable Treg numbers, recipients treated with basiliximab experienced earlier onset of food allergies.
Conclusions:
- CD25 is not essential for the peripheral maintenance of human Tregs in vivo.
- Therapeutic targeting of CD25 may affect Treg functionality, warranting further investigation.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...