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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

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Updated: Jun 10, 2026

A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow
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Hypoxic tumor microenvironments reduce collagen I fiber density.

Samata M Kakkad1, Meiyappan Solaiyappan, Brian O'Rourke

  • 1Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Neoplasia (New York, N.Y.)
|August 7, 2010
PubMed
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Tumor hypoxia alters the collagen I fiber matrix, reducing its density and changing its structure. This extracellular matrix remodeling by hypoxia may affect cancer cell invasion and drug delivery.

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Area of Science:

  • Oncology
  • Biophysics
  • Cancer Biology

Background:

  • Tumor hypoxia is known to influence cancer progression, affecting angiogenesis, apoptosis resistance, and metastasis.
  • The extracellular matrix (ECM), particularly collagen I (Col1) fibers, plays a crucial role in tumor initiation, progression, and invasion.
  • The specific impact of tumor hypoxia on the Col1 fiber matrix is largely unexplored.

Purpose of the Study:

  • To investigate the influence of tumor hypoxia on collagen I fiber density and structure in breast and prostate cancer models.
  • To understand how hypoxia-mediated changes in the ECM might affect cancer cell behavior and therapeutic outcomes.

Main Methods:

  • Utilized second harmonic generation (SHG) microscopy to visualize and quantify Col1 fiber density and volume in hypoxic and normoxic tumor regions.
  • Employed fluorescence microscopy to identify hypoxic regions using enhanced green fluorescent protein (EGFP) reporter systems.
  • Analyzed gene expression using microarrays to identify molecular changes associated with hypoxia in cancer cells.

Main Results:

  • Hypoxic tumor regions showed significantly reduced Col1 fiber density and altered fiber structure compared to normoxic regions.
  • Gene expression analysis revealed increased lysyl oxidase and decreased matrix metalloproteinase expression in hypoxic cancer cells.
  • These findings indicate that hypoxia actively restructures the tumor's collagenous microenvironment.

Conclusions:

  • Tumor hypoxia induces significant remodeling of the collagen I extracellular matrix.
  • Hypoxia-mediated changes in the Col1 fiber matrix may influence cancer cell invasion and the efficacy of macromolecular therapies.
  • Further research into ECM-hypoxia interactions is warranted to develop novel therapeutic strategies.