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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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Salt concentration-induced dehybridisation of DNA-gold nanoparticle conjugate assemblies for diagnostic applications

Ju-Hwan Oh1, Jae-Seung Lee

  • 1Department of Materials Science and Engineering and Institute for Biomedical Research, Korea University, Anam-dong, Seongbuk-gu, Seoul, Republic of Korea 136-713.

Chemical Communications (Cambridge, England)
|August 7, 2010
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...