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Related Experiment Video

Updated: Jun 10, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Quality aspects of TMA analysis.

Pierre Tennstedt¹, Guido Sauter

¹Institute of Pathology, University Medical Center Hamburg Eppendorf, Hamburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|August 7, 2010

Summary

Tissue microarray (TMA) experiment quality depends on every step, from sample preparation to data analysis. Ensuring high standards throughout the complex TMA process is crucial for reliable study outcomes.

Area of Science:

Biopathology
Histology
Clinical Data Analysis

Background:

Tissue microarrays (TMAs) are essential tools in biomedical research.
The reliability of TMA studies hinges on the integrity of each experimental stage.

Purpose of the Study:

To emphasize the critical importance of quality control across all stages of tissue microarray experiments.
To highlight the multifaceted nature of TMA studies, encompassing biological, technical, and analytical components.

Main Methods:

Review of the key stages involved in TMA creation and analysis.
Emphasis on the interconnectedness of sample acquisition, histological assessment, clinical data management, protocol development, and statistical analysis.

Main Results:

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Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

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Published on: June 10, 2022

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Last Updated: Jun 10, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

The overall quality of a TMA experiment is determined by the performance of its weakest link.
Each component, including tissue handling, diagnostic accuracy, data integrity, and analytical rigor, significantly impacts experimental outcomes.

Conclusions:

Maintaining high-quality standards in every phase of tissue microarray experimentation is paramount for generating valid and reproducible research findings.
A holistic approach to quality management is essential for successful TMA studies.