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Related Experiment Video

Updated: Jun 10, 2026

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
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Building "tissue" microarrays from suspension cells.

Shuchun Zhao1, Yasodha Natkunam

  • 1Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2010
PubMed
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This study presents a novel method for creating tissue microarrays (TMAs) from cell suspensions. This technique allows for efficient gene and protein expression analysis from various sample types, including those not suitable for traditional biopsies.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Pathology

Background:

  • Tissue microarrays (TMAs) are essential for high-throughput analysis of RNA or protein expression.
  • Traditional TMAs are typically constructed from paraffin-embedded solid tissues.
  • Analyzing cells in suspension presents unique challenges for TMA construction.

Purpose of the Study:

  • To describe a method for constructing TMAs from small volumes of cells in suspension.
  • To demonstrate the utility of this method for diverse sample types.
  • To enable gene and protein expression studies where traditional biopsies are unavailable.

Main Methods:

  • Cells in suspension (1 x 10^6 to 5 x 10^7) are pelleted after fixation.
  • Processing and embedding are performed within a microcentrifuge tube.

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  • The method preserves cytomorphology and immunoreactivity of fresh and frozen cells.

    • Main Results:

    • Successful construction of TMAs from cell suspensions.
    • Excellent preservation of cellular morphology and antigenicity.
    • Demonstrated applicability to hematopoietic neoplasms, fine needle aspirates, body fluids, and cultured cells.

    Conclusions:

    • This microcentrifuge tube-based TMA method is versatile and efficient for suspension cells.
    • It facilitates gene expression profiling and protein analysis in challenging clinical scenarios.
    • The technique expands the utility of TMAs for research and clinical trials.