Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NTHL1 is a recessive cancer susceptibility gene.

Scientific reports·2023
Same author

Novel ZNF414 activity characterized by integrative analysis of ChIP-exo, ATAC-seq and RNA-seq data.

Biochimica et biophysica acta. Gene regulatory mechanisms·2022
Same author

A search for modifying genetic factors in CHEK2:c.1100delC breast cancer patients.

Scientific reports·2021
Same author

Paclitaxel, Carboplatin and 1,25-D3 Inhibit Proliferation of Ovarian Cancer Cells <i>In Vitro</i>.

Anticancer research·2020
Same author

Recurrent moderate-risk mutations in Finnish breast and ovarian cancer patients.

International journal of cancer·2019
Same author

Correction to: Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells.

BMC cancer·2019

Related Experiment Video

Updated: Jun 10, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

DNA copy number analysis on tissue microarrays.

Anne Kallioniemi1

  • 1Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland. anne.kallioniemi@uta.fi

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2010
PubMed
Summary
This summary is machine-generated.

Detecting DNA copy number changes is crucial for cancer research. Fluorescence in situ hybridization (FISH) combined with tissue microarray (TMA) enables high-throughput genetic aberration analysis in numerous samples.

More Related Videos

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Related Experiment Videos

Last Updated: Jun 10, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • DNA sequence copy number alterations are vital in cancer research and clinical diagnostics.
  • High-throughput analysis of genetic aberrations is needed for large-scale studies.

Purpose of the Study:

  • To evaluate the utility of fluorescence in situ hybridization (FISH) on tissue microarray (TMA) for detecting genetic aberrations.
  • To highlight the challenges and successes of FISH on TMA methodology.

Main Methods:

  • Utilized fluorescence in situ hybridization (FISH) technology.
  • Employed tissue microarray (TMA) for high-throughput sample analysis.
  • Investigated various tissue pretreatment protocols to optimize FISH on TMA.

Main Results:

  • FISH on TMA is a technically demanding but effective method.
  • Successfully uncovered genetic alterations in various malignancies using FISH on TMA.
  • Demonstrated the ability to rapidly establish the clinical significance of genetic changes.

Conclusions:

  • FISH on TMA is a powerful approach for evaluating genetic aberrations in large sample sets.
  • Despite technical challenges, FISH on TMA facilitates cancer research and clinical significance assessment.