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Updated: Jun 10, 2026
Primary Culture of Dental Pulp Stem Cells
Published on: May 5, 2023
Multilineage differentiation of dental pulp stem cells from green fluorescent protein transgenic mice
Brian E Grottkau1, P Prasad Purudappa, Yun-feng Lin
1Department of Orthopaedic Surgery, Mass General Hospital for Children and the Pediatric Orthopaedic Laboratory for Tissue Engineering, Harvard Medical School, Boston, Massachusetts, USA.
Aim:
The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also observed during differentiation.
Methodology:
DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR.
Results:
The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation.
Conclusion:
As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo.
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