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Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
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Characterization of tunable FGF-2 releasing polyelectrolyte multilayers.

Mara L Macdonald1, Natalia M Rodriguez, Nisarg J Shah

  • 1Harvard MIT Division of Health Sciences and Technology and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Biomacromolecules
|August 10, 2010
PubMed
Summary

Novel polyelectrolyte multilayer films enable tunable delivery of fibroblast growth factor 2 (FGF-2) for bone regeneration. These films demonstrate sustained release and preserved FGF-2 bioactivity, paving the way for enhanced tissue engineering applications.

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Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Fibroblast growth factor 2 (FGF-2) is crucial for stem cell functions and bone formation.
  • Current FGF-2 delivery methods face limitations, hindering clinical applications.
  • Polyelectrolyte multilayer (PEM) films offer a promising platform for controlled growth factor delivery.

Purpose of the Study:

  • To explore the loading and release characteristics of FGF-2 from synthetic, degradable PEM films.
  • To investigate the impact of film architecture on FGF-2 loading and release kinetics.
  • To assess the bioactivity of FGF-2 released from PEM films.

Main Methods:

  • Fabrication of hydrolytically degradable PEM films with varying architectures.
  • Tuning FGF-2 loading by adjusting nanolayer number, counterpolyanion, and polycation type.
  • Quantifying FGF-2 loading (7-45 ng/cm²) and release profiles (24 hours to ~5 days).
  • In vitro assessment of released FGF-2's effect on MC3T3 preosteoblast proliferation.

Main Results:

  • FGF-2 loading was tunable based on film design parameters.
  • Release times ranged from 24 hours to approximately five days.
  • Released FGF-2 maintained its bioactivity, promoting preosteoblast proliferation.
  • Biologically derived counterpolyanions (heparin sulfate, chondroitin sulfate) enhanced FGF-2 activity.

Conclusions:

  • PEM films provide a controllable system for FGF-2 delivery.
  • The study demonstrates tunable drug loading and release kinetics for FGF-2.
  • These findings support the potential of PEM films for in vivo bone and tissue regeneration applications.