Related Experiment Video
Updated: Jun 10, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Indian isolates of white spot syndrome virus exhibit variations in their pathogenicity and genomic tandem repeats
K R John1, M R George, T Iyappan
1Department of Aquaculture, Tamilnadu Veterinary & Animal Sciences University, Fisheries College and Research Institute, Tuticorin, India. rijijohn@gmail.com
Abstract:
To detect genomic variation of white spot syndrome virus (WSSV) isolates from different geographical regions of India, the variable number of the tandem repeat (VNTR) region of the ORF 94 (Thailand WSSV isolate - GeneBank Accession No. AF369029) was analysed using five specific sets of primers. Analysis of 70 WSSV-positive samples showed the presence of 14 different genotypes of WSSV with VNTRs ranging from 2 to 16 tandem repeats with the majority (85.47%) having 6-12 tandem repeats. Occurrence of different genotypes of WSSV was found to be neither correlated to any specific geographical region nor to the different growth stage of the tiger shrimp, Penaeus monodon. Pathogenicity studies conducted with 25 isolates of WSSV revealed the presence of virulent and avirulent strains of WSSV in Indian shrimp farms. However, an unambiguous link could not be established between the different genotypes and their virulence.
Related Concept Videos
Viral Recombination
Single Nucleotide Polymorphisms-SNPs
Subviral Agents
Viral Mutations
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Viruses of Archaea
