A multi-target screening analysis in human plasma using fast liquid chromatography-hybrid tandem mass spectrometry
V Viette1, D Guillarme, R Mylonas
1Department of Genetics and Laboratory Medicine, Geneva University Hospital, Switzerland.
Objectives:
Evaluate a new LC-MS/MS screening method for drugs and drugs of abuse as an alternative to the existing methods used in clinical toxicology laboratories.
Design And Methods:
The work was divided in two parts. The first part was dedicated to the technical development and evaluation of the method for which a set of 97 drugs and relevant metabolites was used to perform a complete investigation of matrix effects and lower limit of identification (LOI). The second part was a comparison of identified drugs between LC-MS/MS and Remedi® instrument on clinical serum samples.
Results:
The method offers good performance allowing an automatic peak detection and compound identification. The limit of identification is equivalent to 50 μg/L for the majority of the studied compounds. The process efficiency (PE) is higher than 70% for 65% of the evaluated compounds. Thus, a sufficient detection capability in terms of limit of detection for identification and PE satisfied the expected performance.
Conclusion:
The described methodology allows the identification of the main drugs incriminated in intoxications within a quite short analysis time. The separation of most of the analytes is performed in 15 min. The procedure is sufficiently sensitive and selective.
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