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Updated: Jun 10, 2026

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
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Published on: May 19, 2017

Calcium-responsive paramagnetic CEST agents.

Goran Angelovski1, Thomas Chauvin, Rolf Pohmann

  • 1Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Spemannstr. 38, 72076 Tübingen, Germany. goran.angelovski@tuebingen.mpg.de

Bioorganic & Medicinal Chemistry
|August 10, 2010
PubMed
Summary
This summary is machine-generated.

Novel magnetic resonance imaging agents detect calcium (Ca2+) and magnesium (Mg2+) ions. These chemical exchange saturation transfer (CEST) agents show decreased signal upon ion binding, enabling monitoring of brain activity.

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Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

Area of Science:

  • Biomedical Engineering
  • Radiochemistry
  • Neuroscience

Background:

  • Monitoring extracellular calcium (Ca2+) concentration is crucial for understanding brain neuronal activity.
  • Magnetic resonance imaging (MRI) offers a non-invasive approach for such assessments.
  • Novel chemical exchange saturation transfer (CEST) agents are being developed for enhanced MRI sensitivity.

Purpose of the Study:

  • To synthesize and characterize novel lanthanide complexes as Ca2+-responsive CEST agents for MRI.
  • To investigate the effect of Ca2+ and Mg2+ binding on the CEST properties of these novel ligands.
  • To evaluate the potential of these agents for monitoring Ca2+ levels in biomedical research.

Main Methods:

  • Synthesis of tetraamide (L(1)) and bisamide (L(2)) cyclen derivatives with iminodiacetate moieties.
  • Complexation with Ytterbium (Yb3+) and Europium (Eu3+) ions.
  • CEST MRI experiments at 11.7T and 16T to assess agent response to Ca2+ and Mg2+.
  • Determination of metal ion affinity constants and proton exchange rates.

Main Results:

  • Yb(3+) and Eu(3+) complexes of L(1) exhibited significant CEST effects.
  • Calcium and magnesium binding led to a considerable decrease in the CEST effect for YbL(1) and EuL(1) complexes.
  • Low affinity constants (M(-1) range) suggest single iminodiacetate arm coordination.
  • Ca(2+)/Mg(2+) binding was shown to slow down amide proton exchange, diminishing the CEST signal.

Conclusions:

  • Novel Yb(3+) and Eu(3+) complexes based on L(1) are effective Ca2+-responsive CEST agents.
  • The observed decrease in CEST effect upon Ca2+/Mg2+ binding allows for ion concentration monitoring.
  • These PARACEST MRI agents show promise for biomedical research applications in tracking neuronal activity.