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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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Updated: Jun 10, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Induced pluripotent stem cells.

Holm Zaehres1, Jeong Beom Kim, Hans R Schöler

  • 1Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, NRW, Germany.

Methods in Enzymology
|August 10, 2010
PubMed
Summary
This summary is machine-generated.

Scientists can reprogram somatic cells into induced pluripotent stem (iPS) cells using specific transcription factors. This breakthrough offers potential for disease modeling and regenerative medicine therapies.

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Area of Science:

  • Stem cell biology
  • Molecular biology
  • Regenerative medicine

Background:

  • Induced pluripotent stem (iPS) cells are generated from somatic cells.
  • Reprogramming uses specific transcription factors like Oct4, Sox2, Nanog, Lin28, Klf4, and c-Myc.
  • iPS cells have potential in disease modeling and therapeutic applications.

Purpose of the Study:

  • To discuss methods for inducing pluripotency in somatic cells.
  • To highlight the potential of iPS cells in personalized medicine and therapy.

Main Methods:

  • Retroviral expression of key transcription factors (Oct4, Sox2, Nanog, Lin28, Klf4, c-Myc).
  • Exploration of alternative methods including other expression vectors, protein transduction, and small molecules.
  • Application to various mouse and human somatic cell populations.

Main Results:

  • Successful reprogramming of somatic cells into iPS cells is achievable.
  • Multiple methods demonstrate efficiency and effectiveness in inducing pluripotency.
  • Diverse somatic cell types can be utilized for iPS cell generation.

Conclusions:

  • iPS cell technology offers significant promise for patient-specific disease modeling.
  • iPS cells provide a valuable alternative source for pluripotent stem cells in regenerative medicine.
  • Various efficient induction strategies are available for generating iPS cells from different cell sources.