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Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview

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Related Experiment Video

Updated: Jun 10, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Stem cells and DNA damage: persist or perish?

Andrew A Lane1, David T Scadden

  • 1Center for Regenerative Medicine, Cancer Center, Massachusetts General Hospital, Boston, MA 02114, USA. aalane@partners.org

Cell
|August 10, 2010
PubMed
Summary

Hematopoietic stem cells (HSCs) face DNA damage risks. Mouse and human HSCs exhibit opposing responses, choosing either to die or persist after DNA damage.

Area of Science:

  • Stem cell biology
  • Hematopoiesis
  • DNA damage response

Background:

  • Stem cells possess self-renewal and tissue-repopulating capabilities.
  • These essential functions render stem cells susceptible to genotoxic damage.
  • Understanding stem cell fate decisions after DNA damage is crucial for regenerative medicine.

Discussion:

  • Mohrin et al. (2010) and Milyavsky et al. (2010) investigated stem cell responses to DNA damage.
  • Their research focused on hematopoietic stem cells (HSCs) from both mouse and human origins.
  • Contrasting survival strategies were observed between mouse and human HSCs.

Key Insights:

  • Mouse hematopoietic stem cells (HSCs) tend to persist following DNA damage.
  • Human hematopoietic stem cells (HSCs) predominantly undergo apoptosis in response to DNA damage.

More Related Videos

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Related Experiment Videos

Last Updated: Jun 10, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
10:16

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

  • This divergence highlights species-specific mechanisms in stem cell DNA damage response.

    • Outlook:

    • Further research is needed to elucidate the molecular pathways driving these opposing decisions.
    • Understanding these differences could lead to targeted therapies for stem cell-related diseases.
    • Investigating conserved and divergent DNA repair mechanisms in stem cells is warranted.