Related Experiment Video
Updated: Jun 10, 2026
12:48
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Production of protein complexes via co-expression
John J Kerrigan1, Qing Xie, Robert S Ames
1Biological Reagents & Assay Development, Platform Technology & Science, GlaxoSmithKline R&D, 1250 South Collegeville Road, Collegeville, PA 19426, USA.
Protein Expression and Purification
|August 10, 2010
Summary
Co-expressing protein complexes ensures proper folding and function, enabling detailed studies. This method is crucial for understanding protein interactions and cellular processes.
Area of Science:
- Molecular Biology
- Structural Biology
- Biochemistry
Background:
- Cellular processes rely on multi-protein complexes.
- Protein function depends on interacting partners and stoichiometry.
- Context-specific protein studies are essential.
Purpose of the Study:
- To summarize successful protein co-expression cases.
- To discuss partner selection and production strategies.
- To highlight the importance of co-expression for complex studies.
Main Methods:
- Review of literature on protein co-expression.
- Analysis of strategies for producing protein complexes.
- Case studies of successful co-expression.
Main Results:
- Co-expression yields active protein complexes for study.
- Identified critical factors in selecting co-expression partners.
- Outlined strategies for successful protein complex production.
Conclusions:
- Protein co-expression is vital for studying complex function.
- Successful co-expression facilitates biochemical and structural analyses.
- This approach is key to understanding cellular mechanisms.
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