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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Cancer therapy via modulation of micro RNA levels: a promising future

Satya K Kota1, Savithri Balasubramanian

  • 1Institute of Molecular Genetics, CNRS, UMR5535, 1919 route de Mende, 34293 Montpelier Cedex 5, France. satya.kota@igmm.cnrs.fr

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
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