Related Experiment Video
Updated: Jun 10, 2026

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
Published on: May 4, 2016
Effects of some non-genotoxic chemicals on DNA synthesis in rat hepatocytes
M Moret1, J N Lawrence, D J Benford
1Division of Chemical Safety Evaluation, Robens Institute, University of Surrey, Guildford, Surrey GU2 5XH, UK.
Abstract:
The effect of the antihistaminic drug methapyrilene (MP) on DNA synthesis in primary cultures of rat hepatocytes has been compared with the effects of sodium phenobarbitone (PB), clofibric acid (CA), 2-acetylaminofluorene (AAF) and dimethyl sulfoxide (DMSO). The response for all chemicals was dependent on the concentration of epidermal growth factor (EGF) in the culture medium. MP at concentrations between 0.1 and 1 mum stimulated DNA synthesis. PB had a stimulatory effect on DNA synthesis at 0.5-1 mm. A greater increase in DNA synthesis was observed in the absence of EGF in the culture medium, for both chemicals. CA (0.1 mm) increased DNA synthesis in the absence of EGF and inhibited DNA synthesis at concentrations of 10 ng/ml or more. A dose-related increase in DNA synthesis with DMSO was observed in the presence of 10 ng EGF/ml. AAF did not stimulate DNA synthesis and inhibited it in the presence of 10 ng EGF/ml.
Related Concept Videos
Mutagenicity and Carcinogenicity
Inhibitors of Bacterial DNA Synthesis
Drug Toxicity: Dose-Dependent Reactions
Spontaneous and Induced Mutations
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle

