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Updated: Jun 10, 2026

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

In vitro dermal toxicology using skin organ cultures.

A A Rutten¹, J J van de Sandt

¹TNO Food and Nutrition Research, Department of Biological Toxicology, PO Box 360, 3700 AJ Zeist, The Netherlands.

Toxicology in Vitro : an International Journal Published in Association with BIBRA

|August 10, 2010

Summary

This study developed a novel in vitro skin model for toxicity testing, reducing animal use. The model effectively assessed dermal toxicity, percutaneous absorption, and metabolism of various compounds.

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Area of Science:

Toxicology
Dermatology
In Vitro Models

Background:

Need for reliable, cost-effective in vitro alternatives to animal testing for skin toxicity.
Current methods lack quantitative endpoints and may cause animal discomfort.

Purpose of the Study:

Develop and validate an in vitro model using cultured full-thickness skin from multiple species.
Investigate dermal toxicity, percutaneous absorption, and biotransformation of applied compounds.

Main Methods:

Cultured full-thickness skin from rabbit, pig, and human.
Topical application of test compounds.
Assessed cytotoxicity (MTT assay), inflammatory mediator release (HETE's), percutaneous absorption, and metabolism.

Main Results:

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MTT conversion inhibition correlated with irritant dose, indicating cytotoxicity.
Release of 12-HETE (pro-inflammatory) and 15-HETE (anti-inflammatory) observed.
Percutaneous absorption varied by species (pig > human ≈ rabbit); extensive cutaneous metabolism occurred.

Conclusions:

The developed in vitro skin model provides a viable alternative for assessing dermal toxicity, absorption, and metabolism.
The model demonstrates dose-dependent cytotoxicity and inflammatory mediator release, with potential for repair assessment.
Species-specific differences in absorption and metabolism highlight the model's utility in comparative studies.