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Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

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Related Experiment Video

Updated: Jun 10, 2026

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
21:16

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

Published on: July 13, 2009

New epidermal model for dermal irritancy testing.

C L Cannon1, P J Neal, J A Southee

  • 1Microbiological Associates Ltd., Stirling University Innovation Park, Stirling FK9 4NF, UK.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|August 10, 2010
PubMed
Summary
This summary is machine-generated.

This study validates EpiDerm, a human epidermis model, for predicting skin irritation from surfactants. Results show high reproducibility across labs, supporting its use in toxicological assessments.

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An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
10:13

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Published on: August 23, 2015

Area of Science:

  • Dermatology
  • Toxicology
  • In Vitro Testing

Background:

  • Assessing dermal irritation from surfactants is crucial for product safety.
  • Existing methods often lack reproducibility and require animal testing.
  • The EpiDerm model offers a potential in vitro alternative.

Purpose of the Study:

  • To conduct an interlaboratory comparison of the EpiDerm model.
  • To evaluate the model's ability to predict dermal irritation from surfactants and formulations.
  • To assess the reproducibility of the EpiDerm model across different laboratories and shipping conditions.

Main Methods:

  • An interlaboratory comparison using EpiDerm model.
  • Testing a range of anionic and non-ionic surfactants and formulations.
  • Estimating toxicity via MTT conversion using EC(50) and ET(50) protocols.
  • Comparing in vitro results with human clinical data (chamber irritation and repeat handwash tests).

Main Results:

  • High correlation (r=0.977-0.993) between in vitro EpiDerm results and human clinical irritation.
  • Good reproducibility between the manufacturing site and independent laboratories (r=0.84 and r=0.74).
  • The model demonstrated consistent performance after shipping and storage.

Conclusions:

  • The EpiDerm model is a reproducible and reliable in vitro tool for predicting chemical-induced dermal irritation.
  • Its utility is validated across multiple laboratories and shipping conditions.
  • Further investigation is warranted for its application in toxicological safety assessments.