Related Experiment Video

Updated: Jun 10, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Sensitive measurement of quantity dynamics of FLT3 internal tandem duplication at early time points provides

W-C Chou1, H-A Hou2, C-Y Liu3

  • 1Department of Laboratory Medicine; Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University, Taipei.

Annals of Oncology : Official Journal of the European Society for Medical Oncology
|August 10, 2010
PubMed
Abstract

Insights

Minimal residual disease (MRD) levels of FLT3-ITD in acute myeloid leukemia (AML) after chemotherapy are prognostic. Lower FLT3-ITD levels at early time points predict better survival, especially when combined with NPM1 mutations.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Minimal residual disease (MRD) is a key prognostic factor in acute myeloid leukemia (AML).
  • The stability of FLT3-internal tandem duplication (FLT3-ITD) as an MRD marker has been debated due to semi-quantitative methods.
  • Early assessment of FLT3-ITD dynamics using sensitive methods may offer prognostic value.

Purpose of the Study:

  • To investigate the prognostic significance of FLT3-ITD dynamics in AML.
  • To evaluate FLT3-ITD levels at early time points after chemotherapy as predictive markers.
  • To assess the stability of FLT3-ITD in relation to NPM1 mutations.

Main Methods:

  • Quantitative real-time PCR was used to measure FLT3-ITD levels in 493 serial samples from 55 AML patients.
  • FLT3-ITD quantities were analyzed at two early time points: after induction (TP1) and after the first post-induction chemotherapy (TP2).
  • Median follow-up was 64.8 months.

Main Results:

  • Lower FLT3-ITD levels at TP2 correlated with longer overall survival (OS) and disease-free survival (DFS), irrespective of cytogenetic risk.
  • A ≥3 log reduction in FLT3-ITD at TP2 independently predicted better DFS and showed a trend toward better OS.
  • FLT3-ITD disappeared at relapse in 16.7% of patients, notably less frequent in those with mutant NPM1 (0%) compared to wild-type NPM1 (29.4%).

Conclusions:

  • Early FLT3-ITD levels post-chemotherapy provide significant prognostic information in AML.
  • FLT3-ITD is more stable in patients with concurrent NPM1 mutations.
  • Despite occasional disappearance at relapse, FLT3-ITD dynamics are valuable for predicting AML patient outcomes.

More Related Videos

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...