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Sevoflurane binds and allosterically blocks integrin lymphocyte function-associated antigen-1.

Koichi Yuki1, Nathan S Astrof, Clay Bracken

  • 1Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston, and Department of Anaesthesia, Harvard Medical School, Boston, Massachusetts 02115, USA.

Anesthesiology
|August 10, 2010
PubMed
Summary
This summary is machine-generated.

Sevoflurane, like isoflurane, inhibits leukocyte function-associated antigen-1 (LFA-1) by binding to an allosteric site. This anesthetic-induced LFA-1 inhibition may explain immune system modulation during anesthesia.

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Area of Science:

  • Immunology
  • Anesthesiology
  • Molecular Biology

Background:

  • Volatile anesthetics modulate immune cell function through various mechanisms.
  • Isoflurane inhibits leukocyte function-associated antigen-1 (LFA-1) by binding to its allosteric site.
  • The effect of sevoflurane on LFA-1 function remained to be determined.

Purpose of the Study:

  • To investigate whether sevoflurane, similar to isoflurane, allosterically inhibits LFA-1.
  • To elucidate the mechanism of sevoflurane's interaction with LFA-1.

Main Methods:

  • Flow cytometry assessed sevoflurane's effect on LFA-1 binding to intercellular adhesion molecule-1.
  • Ligand-binding assays with purified LFA-1 protein determined direct interaction.
  • Analysis of wild-type versus high-affinity locked LFA-1 distinguished inhibition types.
  • Nuclear magnetic resonance spectroscopy identified sevoflurane's binding site.

Main Results:

  • Sevoflurane significantly inhibited LFA-1 ligand-binding in leukocytes and cell-free assays.
  • Inhibition occurred via an allosteric mechanism, blocking wild-type but not locked high-affinity LFA-1.
  • Nuclear magnetic resonance confirmed sevoflurane binds to the same allosteric cavity as isoflurane.

Conclusions:

  • Sevoflurane inhibits LFA-1 by blocking activation-dependent conformational changes to its high-affinity state.
  • The allosteric inhibition of LFA-1 by sevoflurane is a potential mechanism for anesthetic-induced immunomodulation.