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Updated: Jun 10, 2026

Super-resolution Imaging of the Bacterial Division Machinery
Published on: January 21, 2013
Live-cell super-resolution imaging with trimethoprim conjugates
Richard Wombacher1, Meike Heidbreder, Sebastian van de Linde
1Department of Chemistry, Columbia University, New York, NY, USA.
Abstract:
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.
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