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Related Experiment Video

Updated: Jun 10, 2026

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
09:23

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

ASPM gene expression in medulloblastoma.

Tânia M Vulcani-Freitas1, Najsla Saba-Silva, Andréa Cappellano

  • 1Institute of Pediatric Oncology, Pediatric Department, Federal University of São Paulo, São Paulo, Brazil.

Child'S Nervous System : Chns : Official Journal of the International Society for Pediatric Neurosurgery
|August 10, 2010
PubMed
Summary

The ASPM gene is overexpressed in pediatric medulloblastoma, a common childhood brain tumor. This finding suggests ASPM may be a potential therapeutic target, though no clinical associations were found in this study.

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Area of Science:

  • Neuro-oncology
  • Developmental neurobiology
  • Cancer genetics

Background:

  • Medulloblastomas are common pediatric central nervous system malignant tumors, often treated aggressively with significant side effects.
  • Current treatments like chemotherapy and radiotherapy lack specificity, necessitating novel therapeutic strategies.
  • The ASPM gene, implicated in glioblastoma, shows potential as a molecular target due to its role in cell proliferation.

Purpose of the Study:

  • To investigate ASPM gene expression in medulloblastoma samples.
  • To correlate ASPM expression levels with clinical features of pediatric medulloblastoma patients.

Main Methods:

  • Gene expression analysis using quantitative PCR (qPCR) with SYBR Green in 37 pediatric medulloblastoma samples.
  • Statistical analysis including t-tests, Mann-Whitney tests, and Kaplan-Meier survival analysis.

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Published on: October 3, 2010

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
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Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

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Related Experiment Videos

Last Updated: Jun 10, 2026

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
09:23

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
05:10

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Published on: October 3, 2010

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

Main Results:

  • All analyzed medulloblastoma samples exhibited significant overexpression of the ASPM gene (over 40-fold).
  • No statistically significant association was identified between ASPM overexpression and the clinical parameters of the patients.

Conclusions:

  • ASPM overexpression may disrupt neural stem cell differentiation during central nervous system development.
  • This disruption potentially contributes to the pathogenesis of medulloblastoma, a tumor originating from embryonic cerebellar progenitor cells.