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Updated: Feb 11, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Published on: June 17, 2014
Methods to measure nuclear export of beta-catenin using fixed and live cell assays
Manisha Sharma1, Beric R Henderson
1Westmead Institute for Cancer Research, Westmead Millennium Institute at Westmead Hospital, The University of Sydney, Westmead, NSW, Australia.
Abstract:
beta-Catenin is a multifunctional protein which is overexpressed in several types of cancers. The subcellular location of beta-catenin at the membrane junctions or in the nucleus determines its function in cell adhesion and transcription activation, respectively. The aberrant localization of beta-catenin in the nucleus activates transcription of a number of genes involved in cancer progression. Our laboratory studies intracellular trafficking of beta-catenin with particular emphasis on its movement and regulation in and out of the nucleus. In this chapter, we outline a number of assays to measure the nuclear export of beta-catenin in fixed and live cells.
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