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[Tumor necrosis factor in uterine leiomyomas at various stages of tumor growth]
Małgorzata Wolańska1, Ewelina Taudul, Emilia Bańkowska-Guszczyn
1Zakład Biochemii Lekarskiej Uniwersytet Medyczny w Białymstoku. ma.wolanska@interia.pl
Objectives:
The aim of the study was to evaluate the expression of tumor necrosis factors -alpha and beta (TNF), their receptor and content in human uterine leiomyomas at various stages of tumor growth.
Material And Methods:
Studies were performed on human myometrium and uterine leiomyomas of various weights (small: less than 10 g and large: more than 100 g). Presence of both growth factors and their receptor was detected by Western Immunoblotting technique. The content of TNF-alpha was evaluated by immunoenzymatic method (ELISA).
Results:
Changes in the expression of tumor necrosis factors and their receptor and difference in content of TNF-alpha during the tumor growth were found.
Conclusions:
Myometrium conversion into leiomyoma and an increase in its mass is accompanied by changes in the expression and contents TNF and TNF RI.
