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Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

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Published on: January 19, 2019

Zwitterionic chitosan derivatives for pH-sensitive stealth coating.

Peisheng Xu¹, Gaurav Bajaj, Tyler Shugg

¹Department of Industrial and Physical Pharmacy, and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.

Biomacromolecules

|August 11, 2010

Summary

This summary is machine-generated.

Zwitterionic chitosan coatings prevent protein adsorption on drug carriers. This pH-tunable material offers a stealth surface, enhancing biocompatibility for potential therapeutic applications.

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Area of Science:

Biomaterials Science
Polymer Chemistry
Drug Delivery Systems

Background:

Chitosan is a biocompatible polysaccharide with a cationic nature.
Cationic surfaces are prone to non-specific protein adsorption, limiting their use in biological applications.
Developing effective stealth coatings for drug carriers is crucial for improving therapeutic efficacy.

Purpose of the Study:

To synthesize zwitterionic chitosan derivatives.
To evaluate their potential as a pH-responsive stealth coating for cationic drug carriers.
To investigate the inhibition of protein adsorption and biocompatibility.

Main Methods:

Chitosan was modified by amidation with succinic anhydride to create zwitterionic derivatives.
The isoelectric point of the modified chitosan was tuned by adjusting the conjugation ratio.
Protein adsorption assays and intraperitoneal injection studies were performed.

Main Results:

Succinic anhydride-conjugated chitosan exhibited a tunable isoelectric point (pH 4.9–7.1).
The zwitterionic coating effectively inhibited protein adsorption onto cationic surfaces at physiological pH.
The material demonstrated compatibility with blood components and good tolerability upon injection.

Conclusions:

Zwitterionic chitosan serves as an effective pH-responsive stealth coating.
It prevents protein adsorption on cationic surfaces, enhancing biocompatibility.
This derivative holds promise for advanced drug delivery systems.