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Updated: Jun 10, 2026
Isolation of Small Noncoding RNAs from Human Serum
Published on: June 19, 2014
Detection of microRNAs in dried serum blots
Santosh Kumar Patnaik1, Reema Mallick, Sai Yendamuri
1Department of Thoracic Surgery, Roswell Park Cancer Institute, Buffalo, NY 14203, USA. santosh.patnaik@roswellpark.org
Abstract:
We observed the preservation of microRNAs in unrefrigerated dried serum blots. Preservation was not adversely affected by drying or storing at 37, 45, or 60°C instead of room temperature, but it was harmed when blots were dried incompletely before storage. Preservation of microRNAs in serum was not diminished if, instead of being kept frozen at -80°C, it was stored as dried blots at room temperature for 5 months or at 37°C for 4 weeks. Thus, dried blots can be a convenient and safer way to save, transport, and store serum for microRNA assays.
Insights
Dried serum blots effectively preserve microRNAs (miRNAs) without refrigeration. This method offers a stable and convenient alternative for storing and transporting serum for miRNA analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- MicroRNAs (miRNAs) are crucial biomarkers for various diseases.
- Current methods for serum storage and transport often require refrigeration or freezing, posing logistical challenges.
Purpose of the Study:
- To evaluate the stability and preservation of miRNAs in dried serum blots under various storage conditions.
- To determine if dried serum blots are a viable alternative to traditional frozen storage for miRNA analysis.
Main Methods:
- Serum samples were blotted and dried.
- Dried blots were stored at different temperatures (room temperature, 37°C, 45°C, 60°C) and for varying durations.
- MicroRNA levels were quantified and compared to those stored under standard frozen conditions (-80°C).
Main Results:
- MicroRNA preservation was maintained in dried serum blots stored at room temperature and elevated temperatures (37°C, 45°C, 60°C).
- Incomplete drying prior to storage negatively impacted miRNA preservation.
- Dried blots showed no diminished miRNA levels after 5 months at room temperature or 4 weeks at 37°C compared to -80°C storage.
Conclusions:
- Dried serum blots provide a stable and reliable method for preserving microRNAs.
- This technique simplifies serum sample handling, transport, and storage for miRNA-based assays.
- Dried serum blots offer a convenient and safer alternative to frozen serum, enhancing accessibility for research and diagnostics.
