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Updated: Jun 10, 2026

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
Effect of polymeric substrate on sludge settleability
António M P Martins1, Ozlem Karahan, Mark C M van Loosdrecht
1Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. m.martins@aguasdoalgarve.pt
Using starch, a polymeric substrate, instead of glucose (monomers) prevented and even cured bulking sludge events. This supports a diffusion gradient theory for sludge bulking in wastewater treatment.
Area of Science:
- Environmental Microbiology
- Wastewater Treatment Engineering
Background:
- Bulking sludge is a major challenge in wastewater treatment, often linked to substrate utilization.
- The role of polymeric substrates, like starch, in sludge bulking has been understudied.
- Kinetic selection theory suggests low substrate concentrations cause bulking, while a newer theory emphasizes diffusion-limited gradients.
Purpose of the Study:
- To investigate the effect of a polymeric substrate (starch) on sludge settleability.
- To compare the bulking sludge potential of starch versus monomers (glucose).
- To evaluate the validity of diffusion gradient theory in explaining sludge bulking.
Main Methods:
- Experimental comparison of sludge settleability using starch and glucose as substrates.
- Induction and remediation of bulking sludge events.
- Microscopic observation of sludge floc structure.
Main Results:
- Conditions causing bulking sludge with glucose did not induce bulking with starch.
- Substituting glucose with starch successfully cured an existing bulking sludge event.
- Starch-fed flocs were more porous and open than those fed with maltose or glucose.
Conclusions:
- Results support the diffusion gradient theory for bulking sludge.
- Polymeric substrates like starch may mitigate bulking by reducing substrate gradients within flocs.
- Sludge floc structure is influenced by the type of substrate, with implications for settling properties.
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