Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pro-regenerative fingerprints identified in a sub-population of adult mouse cardiomyocytes by integrative single-cell proteomics.

Genome biology·2026
Same author

Nature of False Peptide Identifications in Data-Independent Acquisition-Based Proteome Analysis.

Journal of the American Society for Mass Spectrometry·2026
Same author

A ginsenoside metabolite and its derivative target PRELID3B against lung cancer cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Peptide-to-Protein Data Aggregation Using Fisher's Method Improves Target Identification in Chemical Proteomics.

Analytical chemistry·2026
Same author

Proteasome Inhibition as a Cancer Type-Specific Off-Target Effect of Everolimus in Cancer Cells.

Biochemistry. Biokhimiia·2026
Same author

Above-Filter Digestion Proteomics Reveals Drug Targets and Localizes Ligand Binding Site.

Journal of proteome research·2026

Related Experiment Video

Updated: Jun 10, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Calibration function for the Orbitrap FTMS accounting for the space charge effect.

Mikhail V Gorshkov1, David M Good, Yaroslav Lyutvinskiy

  • 1Institute for Energy Problems of Chemical Physics, Moscow, Russia. gorshkov@chph.ras.ru

Journal of the American Society for Mass Spectrometry
|August 11, 2010
PubMed
Summary
This summary is machine-generated.

We developed a new calibration law to correct for space-charge effects in Orbitrap mass spectrometry. This method significantly improves mass measurement accuracy for peptides, achieving sub-parts-per-million levels.

More Related Videos

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
10:13

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS

Published on: November 8, 2024

Related Experiment Videos

Last Updated: Jun 10, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
10:13

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS

Published on: November 8, 2024

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Biochemistry

Background:

  • Orbitrap Fourier transform mass spectrometry (FTMS) offers high performance for Life Sciences.
  • Ion cloud interactions in electrostatic traps limit mass measurement accuracy.
  • Space-charge effects cause systematic errors and interference in Orbitrap FTMS.

Purpose of the Study:

  • To characterize space-charge effects on measured frequencies in Orbitrap FTMS.
  • To improve mass measurement accuracy (MMA) for peptides.
  • To develop a calibration law accounting for ion population.

Main Methods:

  • Characterization of space-charge effects on Orbitrap FTMS frequencies.
  • Development of an m/z calibration law based on total ion population.
  • Validation of the calibration law across a wide range of ion populations.

Main Results:

  • Achieved a zero-space charge MMA limit of 80 ppb for a commercial Orbitrap FTMS.
  • Demonstrated sub-ppm MMA over a broad range of ion populations (AGC 10 to 10^7).
  • The proposed calibration law effectively corrects for space-charge induced errors.

Conclusions:

  • The developed m/z calibration law significantly enhances Orbitrap FTMS accuracy.
  • Sub-ppm mass measurement accuracy is achievable for peptides even with varying ion populations.
  • This work addresses a key limitation in Orbitrap FTMS performance.