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Updated: Jun 10, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
MASiVE: Mapping and Analysis of Sirevirus Elements in plant genome sequences
N Darzentas1, A Bousios, V Apostolidou
1Institute of Agrobiotechnology, Centre for Research and Technology Hellas, Thessaloniki 57001, Greece. ndarz@certh.gr
Unlabelled:
We present MASiVE, an expertly built tool for the large-scale, yet sensitive and highly accurate, discovery, preliminary analysis and insertion age estimation of intact Sirevirus LTR-retrotransposons in plant genomic sequences. Validation was based on the recently available and annotated large maize chromosome one. Results show a considerable improvement in the annotation of Sireviruses, and support our approach as an important addition to the bioinformatics toolbox of plant biologists.
Availability:
PERL source code and essential files are available online at http://bat.ina.certh.gr/tools/masive/. The freely available Vmatch, LTRharvest, Wise2, and MAFFT algorithms are required.
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