Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Histone Variants at the Centromere

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

Meiosis II

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Limited Contribution of T<sub>1</sub> Relaxation to GluCEST MRI Signal Differences Across Four Rat Models with Distinct Pathophysiological Features.

Molecular imaging and biology·2026

Same author

Time-resolved GluCEST MRI of acute glutamate-related signal changes following kainic acid administration.

Journal of the neurological sciences·2026

Same author

BPTF regulates androgen receptor activity by enhancing chromatin accessibility and stabilizing the AR-FOXA1 interaction.

Nature communications·2025

Same author

Amide Proton Transfer-Weighted MR Imaging and Signal Variations in a Rat Model of Lipopolysaccharide-Induced Sepsis-Associated Encephalopathy.

Metabolites·2025

Same author

PICH impacts the spindle assembly checkpoint via its DNA translocase and SUMO-interaction activities.

Life science alliance·2025

Same author

USP11 promotes prostate cancer progression by up-regulating AR and c-Myc activity.

Proceedings of the National Academy of Sciences of the United States of America·2024

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation.

Hyunju Ryu¹, Yoshiaki Azuma

¹Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA.

The Journal of Biological Chemistry

|August 11, 2010

Summary

The N-terminal domain of PIASy is crucial for its centromeric localization and mitotic SUMOylation. This localization is mediated by interaction with the Rod/Zw10 complex, ensuring proper chromosomal protein modification during mitosis.

More Related Videos

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Published on: July 18, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Related Experiment Videos

Last Updated: Jun 10, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Published on: July 18, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

SUMO conjugation is vital for mitosis.
PIASy (Protein Inhibitor of Activated STAT y) is a SUMO E3 ligase essential for mitotic SUMOylation of chromosomal proteins.
The regulatory mechanism of PIASy-dependent SUMOylation during mitosis remains unclear.

Purpose of the Study:

To elucidate the regulatory mechanism of PIASy-dependent SUMOylation during mitosis.
To identify the domains of PIASy responsible for its function in mitosis.
To determine the interaction partners of PIASy involved in centromeric localization and SUMOylation.

Main Methods:

Utilized truncated PIASy proteins to assess domain function.
Employed Xenopus egg extracts for in vitro SUMO modification assays.
Performed protein interaction studies and immunofluorescence to determine localization and colocalization.
Conducted experiments involving depletion of specific proteins (Rod) to assess functional impact.

Main Results:

The N terminus of PIASy is essential for mitotic SUMOylation in Xenopus egg extracts, though not for in vitro SUMO modification.
Swapping the N terminus with other PIAS family members disrupted chromosomal binding and mitotic SUMOylation.
The N-terminal domain of PIASy is sufficient for centromeric localization and interacts with the Rod/Zw10 complex.
Depletion of Rod compromised centromeric localization of PIASy and SUMO2/3 during mitosis.

Conclusions:

PIASy utilizes its N-terminal domain to localize to the centromeric region during mitosis.
Interaction with the Rod/Zw10 complex is critical for PIASy's centromeric localization and subsequent execution of centromeric SUMOylation.
This study reveals a fundamental mechanism for PIASy-mediated centromeric SUMOylation in mitosis.