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Updated: Jun 10, 2026

Minimally-invasive Technique for Injection into Rat Optic Nerve

Minimally-invasive Technique for Injection into Rat Optic Nerve

Published on: May 19, 2015

Optic nerve regeneration.

Larry I Benowitz¹, Yuqin Yin

¹F.M. Kirby Neurobiology Center, Children's Hospital Boston, Department of Surgery, Harvard Medical School, Massachusetts, USA. larry.benowitz@childrens.harvard.edu

Archives of Ophthalmology (Chicago, Ill. : 1960)

|August 11, 2010

Summary

This summary is machine-generated.

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Neurogenesis and Regeneration of Nervous Tissue

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In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

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In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration.

Methods in molecular biology (Clifton, N.J.)·2024

Retinal ganglion cell axons typically do not regenerate after injury, causing vision loss. Recent advances in animal models show promising strategies to stimulate axon regrowth, offering hope for future human therapies.

Area of Science:

Neuroscience
Ophthalmology
Regenerative Medicine

Background:

Retinal ganglion cells (RGCs) in the eye usually cannot regenerate their axons after injury or disease.
This lack of regeneration leads to permanent vision loss.
Current research focuses on overcoming these limitations.

Purpose of the Study:

To explore methods for stimulating RGC axon regeneration.
To investigate strategies for enhancing RGC survival and overcoming inhibitory environments.
To assess the potential for clinically meaningful RGC regeneration.

Main Methods:

Activating the intrinsic growth state of RGCs.
Maintaining RGC viability through specific interventions.
Counteracting inhibitory signals in the extracellular environment.

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Transconjunctival Approach for Injection into the Rat Optic Nerve

Transconjunctival Approach for Injection into the Rat Optic Nerve

Published on: April 4, 2025

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Last Updated: Jun 10, 2026

Minimally-invasive Technique for Injection into Rat Optic Nerve

Minimally-invasive Technique for Injection into Rat Optic Nerve

Published on: May 19, 2015

Transconjunctival Approach for Injection into the Rat Optic Nerve

Transconjunctival Approach for Injection into the Rat Optic Nerve

Published on: April 4, 2025

Utilizing advanced animal models to test regenerative approaches.

Main Results:

Significant progress has been made in achieving RGC axon regeneration in animal models.
Strategies to activate intrinsic growth and improve the extracellular environment show partial reversal of vision loss.
The extent of regeneration achievable has been progressively extended.

Conclusions:

Activating RGC intrinsic growth and modifying the environment are key to axon regeneration.
Advances in animal models provide a strong foundation for developing clinical therapies.
Guiding regenerating axons to their correct targets is crucial for restoring functional vision.