Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Monte Carlo simulation for speckle reduction by a moving diffuser on the natural test image.

Applied optics·2016

Same author

Simulating the human eye in measurements of speckle from laser-based projection displays.

Applied optics·2014

Same author

Versatile method for achieving 1% speckle contrast in large-venue laser projection displays using a stationary multimode optical fiber.

Optics express·2012

Same author

Cytotype regulation of P transposable elements in Drosophila melanogaster: repressor polypeptides or piRNAs?

Genetics·2008

Same author

Efficient 2(nd) and 4(th) harmonic generation of a single-frequency, continuous-wave fiber amplifier.

Optics express·2008

Same author

Speckle with a finite number of steps.

Applied optics·2008

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Very efficient speckle contrast reduction realized by moving diffuser device.

Shigeo Kubota¹, Joseph W Goodman

¹Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. kubota@iis.u-tokyo.ac.jp

|August 11, 2010

Summary

This summary is machine-generated.

A novel test bench precisely measures speckle contrast for noise reduction evaluation. Temporal averaging with a vibrating diffuser significantly reduced speckle contrast, demonstrating an effective noise reduction method.

More Related Videos

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Published on: December 2, 2017

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Related Experiment Videos

Last Updated: Jun 10, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Published on: December 2, 2017

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Area of Science:

Optics and Photonics
Image Processing

Background:

Speckle noise degrades image quality in coherent imaging systems.
Accurate measurement of speckle contrast is crucial for evaluating noise reduction techniques.

Purpose of the Study:

To develop and utilize a precise test bench for speckle contrast measurement.
To evaluate the efficiency of temporal averaging for speckle noise reduction.

Main Methods:

A specialized test bench capable of measuring high (100%) and low (<10%) speckle contrast was employed.
Temporal averaging was achieved using a diffuser mounted on a tuning fork vibrating at 100 Hz with a 60 micrometer amplitude.
The diffuser's vibration amplitude was three times the surface roughness correlation length.

Main Results:

The test bench successfully measured speckle contrast across a wide dynamic range.
Significant speckle contrast reduction was achieved through temporal averaging.
The method demonstrated high efficiency in reducing speckle noise.

Conclusions:

A precise speckle contrast measurement bench is essential for robust evaluation of noise reduction.
Temporal averaging using a vibrating diffuser is a highly effective technique for speckle noise reduction.
The experimental setup validates the efficacy of dynamic diffusers for speckle mitigation.