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High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
Published on: September 15, 2021
Flow cytometric analysis of deep-seated lymph nodes
Zorana Miletić1, Branimir Gizdić, Tajana Stoos-Veić
1Department of Clinical Cytology and Cytometry, Dubrava University Hospital, Zagreb, Croatia. zorana.miletic@kbd.hr
Collegium Antropologicum
|August 12, 2010
Summary
Endoscopic ultrasound-guided fine needle aspiration with flow cytometry is effective for diagnosing deep-seated non-Hodgkin
Area of Science:
- Oncology
- Hematology
- Diagnostic Pathology
Background:
- Flow cytometry (FC) immunophenotyping is crucial for evaluating lymphadenopathy and diagnosing non-Hodgkin's lymphomas (NHLs).
- Detection of immunoglobulin light-chain restriction is standard for confirming B-cell NHL monoclonality.
Purpose of the Study:
- To assess the utility of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for FC analysis of deep-seated lymph nodes.
- To compare FC clonality analysis results with cytomorphologic diagnoses.
Main Methods:
- EUS-FNA was used to obtain samples from 16 patients with suspected deep-seated NHL.
- Four-color multiparameter FC analysis (kappa/lambda/CD19/CD45) was performed on 11 samples for clonality assessment.
- Cytological diagnosis included direct smears and rapid on-site evaluation.
Main Results:
- Monoclonality was confirmed by FC in 7 of 11 cases diagnosed cytologically as NHL.
- Four of 11 cases diagnosed as benign were found to be polyclonal by FC.
- EUS-FNA combined with FC demonstrated high sensitivity and specificity for diagnosing deep-seated B-NHL.
Conclusions:
- EUS-FNA with FC is a sensitive and specific method for diagnosing deep-seated B-cell non-Hodgkin's lymphomas.
- Combining cytologic diagnosis with FC clonality analysis can often obviate the need for open biopsy.
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