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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

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Published on: July 27, 2018

Minimizing tissue damage in electroosmotic sampling.

Amy E Hamsher1, Hongjuan Xu, Yifat Guy

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA.

Analytical Chemistry
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

Electroosmotic sampling offers a new way to collect extracellular fluid from tissues. Minimizing electrical power below 120 microW during sampling is key to preventing tissue damage in organotypic hippocampal slice cultures.

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Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Tissue Engineering

Background:

  • Electroosmotic sampling utilizes electric fields to draw extracellular fluid into capillaries.
  • Organotypic hippocampal slice cultures (OHSCs) are used as a model tissue.
  • Minimizing tissue damage is crucial for the viability of electroosmotic sampling.

Purpose of the Study:

  • To define conditions for electroosmotic sampling that minimize damage to OHSCs.
  • To assess the relationship between electrical parameters and tissue damage.
  • To optimize electroosmotic sampling for biological applications.

Main Methods:

  • Electroosmotic sampling performed using varying capillary diameters, tip-tissue distances, and applied voltages.
  • Tissue damage assessed via propidium iodide fluorescence 16-24 hours post-sampling.
  • Electrical power dissipated in the tissue calculated (current x potential drop).

Main Results:

  • Tissue damage was found to be negligible when power was below 120 microW.
  • Smaller capillary inner diameters, lower voltages, and increased tip-tissue distances reduced power.
  • These parameters collectively contribute to minimizing tissue damage during sampling.

Conclusions:

  • Electroosmotic sampling can be performed with minimal tissue damage under specific conditions.
  • Controlling electrical power is the primary factor in preventing damage to OHSCs.
  • Optimized parameters enable safer and more effective extracellular fluid collection from delicate tissues.