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Updated: Dec 31, 2025

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Lentivirus transgenesis.

Alexander Pfeifer¹, Tiongti Lim, Katrin Zimmermann

¹Institute of Pharmacology and Toxicology, Biomedical Center (BMZ), University of Bonn, Germany.

Methods in Enzymology

|August 12, 2010

Summary

This summary is machine-generated.

Lentiviral transgenesis offers a versatile method for creating transgenic animals across various species. This protocol details lentiviral vector use in early embryos and integrant analysis.

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Published on: January 12, 2018

Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

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Last Updated: Dec 31, 2025

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

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Published on: January 12, 2018

Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Area of Science:

* Molecular Biology
* Genetics
* Developmental Biology

Background:

* Traditional microinjection for creating transgenic animals is limited to specific mouse strains.
* Lentiviruses, complex retroviruses, integrate their genetic material into host chromosomes.
* Lentiviral vectors show high efficiency in transferring transgenes into oocytes and early embryos.

Purpose of the Study:

* To present a detailed protocol for generating transgenic animals using lentiviral transduction of early embryos.
* To describe methods for analyzing viral integrants within the genomes of resulting transgenic animals.
* To establish lentiviral transgenesis as a broadly applicable technique across multiple species.

Main Methods:

* Transduction of early embryos with lentiviral vectors carrying specific transgenes.
* Culturing and development of transduced embryos to generate transgenic offspring.
* Molecular analysis techniques to detect and confirm viral DNA integration in transgenic animals.

Main Results:

* Successful generation of transgenic animals using the lentiviral transduction protocol.
* Demonstration of efficient gene transfer and integration in various species including mice, rats, pigs, and non-human primates.
* Validation of methods for analyzing viral integrants, confirming successful genetic modification.

Conclusions:

* Lentiviral transgenesis provides a robust and widely applicable alternative to traditional methods.
* The presented protocol facilitates the efficient generation of transgenic animals across diverse species.
* This technique expands the possibilities for genetic manipulation and research in various animal models.