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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Updated: Jun 10, 2026

Principles of Site-Specific Recombinase (SSR) Technology
07:06

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Genetic fate mapping using site-specific recombinases.

Emilie Legué1, Alexandra L Joyner

  • 1Memorial Sloan-Kettering Cancer Center, New York, USA.

Methods in Enzymology
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

Genetic fate mapping in mice uses site-specific recombinases to track cell development and regeneration. This technique allows researchers to follow cell lineages, aiding the study of organogenesis and stem cell integration.

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Last Updated: Jun 10, 2026

Principles of Site-Specific Recombinase (SSR) Technology
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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
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Published on: November 12, 2012

Area of Science:

  • Developmental biology
  • Cell biology
  • Genetics

Background:

  • Understanding cellular assembly in 3D for organ and organism generation is crucial.
  • Studying adult stem cell integration during regeneration and injury response is vital.
  • Analyzing distinct cell type behaviors during development and regeneration requires effective techniques.

Purpose of the Study:

  • To review genetic fate mapping methods for studying cell behaviors.
  • To detail the tools and practical considerations for these techniques.
  • To advance the understanding of cellular processes in development and regeneration.

Main Methods:

  • Utilizing transgenic mice and site-specific recombinases (SSRs) for genetic fate mapping.
  • Employing inducible SSRs and reporter alleles for sophisticated lineage tracing.
  • Reviewing intrachromosomal (cumulative, inducible, clonal, intersectional) and interchromosomal recombination methods.

Main Results:

  • Genetic fate mapping allows indelible marking of precursor cells and their descendants.
  • Inducible SSRs and reporter alleles enable precise temporal and spatial control of cell marking.
  • Various genetic fate mapping strategies offer different capabilities for lineage analysis.

Conclusions:

  • Genetic fate mapping is fundamental for studying cell proliferation, movement, and lineage segregation.
  • Advanced genetic fate mapping techniques in mice provide powerful tools for developmental and regenerative biology research.
  • Careful consideration of methods, tools, and practicalities is essential for successful fate mapping studies.