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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 10, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Gene trap mutagenesis in the mouse.

Roland H Friedel1, Philippe Soriano

  • 1Department of Neurosurgery, Mount Sinai School of Medicine, New York, USA.

Methods in Enzymology
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

Gene trapping in mouse embryonic stem (ES) cells offers efficient genome mutagenesis, disrupting gene function and identifying insertion sites. This review covers gene trapping applications and provides protocols for retroviral and transposon vectors.

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Published on: April 2, 2014

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11:48

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Published on: July 24, 2013

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Gene trapping is a powerful technique for large-scale functional genomics in mammals.
  • It enables gene disruption, expression monitoring, and site identification within the genome.
  • Advancements include site-specific recombination for allele modification and pathway screening.

Purpose of the Study:

  • To review diverse applications of gene trapping technology.
  • To detail experimental protocols for gene trapping in mouse ES cells.
  • To highlight recent developments in gene trapping strategies.

Main Methods:

  • Gene trap vector delivery via plasmid electroporation, retroviral infection, or transposon-mediated insertion.
  • Utilizing site-specific recombination for in vitro and in vivo trap allele modification.
  • Developing gene trapping strategies for screening specific biological pathways.

Main Results:

  • Gene trapping efficiently disrupts gene function and reports expression patterns.
  • Provides a tag for precise identification of vector insertion sites.
  • Enables screening of genes involved in specific biological processes.

Conclusions:

  • Gene trapping in mouse ES cells is a versatile tool for mammalian genome mutagenesis.
  • Recent technological advancements enhance its utility for genetic studies.
  • Detailed protocols facilitate the application of retroviral and transposon-based gene trapping.