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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Gene Trapping Using Gal4 in Zebrafish
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A wider context for gene trap mutagenesis.

Joshua M Brickman1, Anestis Tsakiridis, Christine To

  • 1MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.

Methods in Enzymology
|August 12, 2010
PubMed
Summary

Gene trapping technology aids in gene identification and mutation using embryonic stem (ES) cells. This review explores gene trap vector applications for transcriptome annotation and cell-based analyses.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Genomics

Background:

  • Gene trapping, a method for gene identification and mutation, was initially developed for random integration in embryonic stem (ES) cells.
  • Large-scale gene trapping initiatives have generated a substantial collection of mutant ES cell lines and mice.
  • Gene trap vector elements are integrated into gene targeting vectors to enhance homologous recombination efficiency.

Purpose of the Study:

  • To review the current state of gene trapping technology.
  • To analyze the applications of specific gene trap vector types.
  • To assess the utility of gene trap vectors for transcriptome annotation and cell-based studies.

Main Methods:

  • Review of existing literature on gene trapping technology and vector applications.
  • Analysis of gene trap vector behavior in various experimental contexts.
  • Consideration of data from large-scale gene trapping initiatives.

Main Results:

  • Gene trapping remains a valuable tool, contributing significantly to mutant cell line and mouse generation.
  • Specific gene trap vector designs influence their application and efficiency.
  • Gene trap vectors provide insights into mammalian transcriptome annotation.

Conclusions:

  • Gene trapping technology continues to be relevant for genetic research and functional genomics.
  • Gene trap vectors serve as versatile tools for cell-based expression analysis and differentiation screening.
  • The application of gene trap vectors extends to various cell lineages, aiding in diverse biological studies.