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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jun 10, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Gene knockdown in the mouse through RNAi.

Aljoscha Kleinhammer1, Wolfgang Wurst, Ralf Kühn

  • 1Institute for Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Munich, Germany.

Methods in Enzymology
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

This study presents a novel method for gene knockdown in mice using short hairpin RNAs (shRNAs) for precise gene function analysis. This approach enables body-wide, cell-specific, or inducible gene silencing for efficient in vivo research.

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Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

Area of Science:

  • Molecular Biology
  • Genetics
  • Mammalian Cell Biology

Background:

  • RNA interference (RNAi) is a standard technique for gene function studies in cell cultures.
  • Existing methods for gene knockdown in mice, such as knockout approaches, can be time-consuming.
  • Short hairpin RNAs (shRNAs) offer a faster alternative for gene silencing in vivo.

Purpose of the Study:

  • To develop a reproducible strategy for gene knockdown in mice using shRNAs.
  • To enable cell type-specific and inducible gene silencing.
  • To facilitate in vivo assessment of gene function.

Main Methods:

  • Utilizing Cre recombinase or doxycycline to control shRNA expression.
  • Integrating shRNA vectors into the Rosa26 locus of ES cells via recombinase-mediated cassette exchange.
  • Generating chimeric mice to transmit vectors through the germ line.
  • Employing site-specific insertion of single-copy shRNA vectors.

Main Results:

  • Achieved reproducible expression of shRNAs in mice.
  • Demonstrated the ability to elicit body-wide, cell type-specific, or inducible gene silencing.
  • Established a method for the expedited production of knockdown mice.

Conclusions:

  • The described strategy provides a robust and efficient system for in vivo gene knockdown in mice.
  • This technique simplifies the assessment of gene function in living organisms.
  • The use of Rosa26 locus insertion and inducible systems enhances the precision and applicability of RNAi in mouse models.